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KMID : 0360220090500060919
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Journal of the Korean Ophthalmological Society
2009 Volume.50 No. 6 p.919 ~ p.922
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Effect of Nitric Oxide on the Survival of R28 Cells
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Lee Jeong-Il
Kim Jae-Woo
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Abstract
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Purpose: To evaluate the effect of nitric oxide (NO) on the survival of R28 cells.
Methods: After immunostaining for GFAP, vimentin, S-100, and neurofilament, R28 cells were exposed to S-nitroso-N-acetyl-D, L-penicillamine (SNAP) at various concentrations, with and without the NO inhibitor, Nω-Nitro-L-arginine methyl ester (L-NAME) for 1 and 3 days. Cellular survival of R28 cells and the production of NO were quantified by rapid colorimetric assays using the MTT and Griess assay, respectively. To evaluate the effect of serum, 10% serum or serum-free media were used separately.
Results: R28 cells showed strong immunoreactivity to GFAP and vimentin compared to S-100 or neurofilament. SNAP inhibited the survival of R28 cells in a dose-dependent manner, and this effect of NO on the cellular survival was abolished by L-NAME. These results were similar after exposure for 1 and 3 days, regardless of the presence of serum in the media.
Conclusions: The current results suggest that NO decreased the survival of R28 cells. Further studies are necessary to evaluate the mechanism of cytotoxicity of the R28 cells.
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KEYWORD
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Neurotoxicity, Nitric oxide, R28 cells
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